Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology

Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology.

Proteome analysis is most commonly accomplished by a combination of two-dimensional gel electrophoresis (2DE) to separate and visualize proteins and mass spectrometry (MS) for protein identification. Although this technique is powerful, mature, and sensitive, questions remain concerning its ability to characterize all of the elements of a proteome. In the current study, more than 1,500 features...

Two-dimensional Gel Electrophoresis

Proteome evaluation of human cystic echinococcosis sera using two dimensional gel electrophoresis

Aim Detection of protein expression changes in human cystic echinococcosis sera by 2D gel electrophoresis. Background Diagnosis and successful treatment of cystic echinococcosis (CE) is a major challenge, up to now. Identification of related expressed proteins using proteomics tools and bioinformatics analysis of patients' sera have not been investigated, so far. Methods Sera from eight con...

Analysis of Piper sarmentosum proteome using two dimensional gel electrophoresis and mass spectrometry

* Author for correspondence: Professor Lay-Harn Gam, School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia. Tel.: 604-6532208, Fax: 604-6570017, Email [email protected]. Abstract. P. sarmentosum is a traditionally used medicinal plant. Protein profiling of leaf extracts of P. sarmentosum was performed using two-dimensional electrophoresis (2-DE). The 2-DE patter...

Rice Proteome Database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003

The Rice Proteome Database is the first detailed database to describe the proteome of rice. The current release contains 21 reference maps based on two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) of proteins from rice tissues and subcellular compartments. These reference maps comprise 11 941 identified proteins showing tissue and subcellular localization, corresponding to 4180 sepa...